糖类及糖代谢

BTP-糖类及糖代谢途径相关物质分析

糖代谢在很多生化过程中都扮演了重要的作用。那么什么是糖类呢，wikipedia对糖的定义是多羟基的醛或酮及其衍生物的有机化合物，可以以直链或环的形式存在。糖类物质的基本组成单位包括半乳糖、果糖以及十分重要的葡萄糖等单糖物质，在能量的储存和运输以及作为结构性组分在生命过程中扮演着重要的角色。百泰派克公司采用以GC/MS(7890A/5975C)和ACQUITY UPLC/TripleQuad5500（Waters/AB Sciex）两大平台联用，从而实现多类糖类物质及代谢相关的糖类物质，进行定量及定性分析，其中涉及到的95%的物质均使用标准品和同位素标准品定量。

机体内糖类物质的代谢途径主要包括葡萄糖的无氧酵解、有氧氧化、磷酸戊糖途径，糖醛酸途径、多元醇途径、糖原合成与糖原分解、糖异生以及其他己糖代谢等。如，其中糖酵解主要的生理功能是在缺氧时迅速提供能量。三羧酸循环，也称柠檬酸循环，是糖、脂肪和蛋白质等物质在体内被彻底氧化的共同代谢途径。

BTP可检测糖类及糖代谢途径相关物质

百泰派克的技术员们经过多年的技术积累，采用ACQUITY UPLC/TripleQuad5500（Waters/AB Sciex）结合糖类及糖代谢途径相关物质标准品，配合糖类及糖代谢途径相关物质同位素标准品进行定量及定性分析：

糖类物质分析服务列表（14 种物质，Low-MW sugars） Quantification of ribose, xylose, ribulose, arabinose, rhamnose, xylulose, fucose, glucose, mannose, galactose, fructose, maltose, lactose, cellobiose by UPLC-MRM/MS
1	Arabinose
2	Cellobiose
3	Fructose
4	Fucose
5	Galactose
6	Glucose
7	Lactose
8	Maltose
9	Mannose
10	Rhamnose
11	Ribose
12	Ribulose
13	Xylose
14	Xylulose

糖酵解 / 三羧酸循环 / 核苷酸（中心代谢途径）分析服务列表 (20 种物质，Glycolysis / TCA / Nucleotide analysis (Central Metabolism Profile)) Profile of Central Metabolism, including glycolysis, pentose-phosphate shunt, TCA cycle and nucleotide pools. All analytes are measured by ESI ionization on a LC-QTOF mass spectrometer.
1	Acetyl-CoA
2	Citrate/Isocitrate combined
3	Succinate
4	Malate
5	2-Phosphoglycerate/3-Phosphoglycerate combined
6	Phosphoenolpyruvate
7	Adenosine monophosphate
8	Adenosine diphosphate
9	Adenosine triphosphate
10	Flavin adenine dinucleotide
11	Nicotinamide adenine dinucleotide
12	Nicotinamide adenine dinucleotide, reduced
13	Nicotinamide adenine dinucleotide phosphate
14	Nicotinamide adenine dinucleotide phosphate, reduced
15	Erythrose 4-phosphate
16	Ribulose 5-phosphate / Xylulose 5-phosphate / ribose-5-phosphate combined
17	6-phosphogluconate
18	Sedoheptulose 7-phosphate
19	Fructose-6-phosphate + glucose-6-phosphate
20	Fructose-bisphosphate

糖酵解 / 三羧酸 / NAD 等相关物质分析服务列表（25 种物质，Gly/TCA/nucleotide and NAD related metabolites） Profile of Central Metabolism, including glycolysis, pentose-phosphate shunt, TCA cycle, nucleotide pool, and NAD related metabolites. All analytes and Internal Standards are measured by ESI ionization on a LC-QTOF mass spectrometer.
1	Acetyl-CoA
2	Citrate/Isocitrate combined
3	Succinate
4	Malate
5	2-Phosphoglycerate/3-Phosphoglycerate combined
6	Phosphoenolpyruvate
7	Adenosine monophosphate
8	Adenosine diphosphate
9	Adenosine triphosphate
10	Flavin adenine dinucleotide
11	Nicotinamide adenine dinucleotide

12	Nicotinamide adenine dinucleotide, reduced
13	Nicotinamide adenine dinucleotide phosphate
14	Nicotinamide adenine dinucleotide phosphate, reduced
15	Erythrose 4-phosphate*
16	Ribulose 5-phosphate / Xylulose 5-phosphate / ribose-5-phosphate combined*
17	6-phosphogluconate*
18	Sedoheptulose 7-phosphate*
19	Fructose-6-phosphate + glucose-6-phosphate
20	Fructose-bisphosphate
21	Nicotinic acid (NA)
22	Nicotinic acid mononucleotide (NaMN)
23	Quinolinic acid (QA)
24	nicotinamide mononucleotide (NMN)
25	nicotinamide (NAM)

三羧酸循环分析服务列表（58 种物质，TCA-Plus，(Central Metabolism Profile)) Profile of Central Metabolism, including glycolysis, pentose-phosphate shunt, TCA cycle, and nucleotide pools by ESI ionization on a LC-QTOF MS
1	Acetyl-CoA
2	Citrate/Isocitrate combined
3	Succinate
4	Malate
5	2-Phosphoglycerate/3-Phosphoglycerate combined 2PG/3PG C3H7O7P 1 PEP C3H5O6P 1
6	Phosphoenolpyruvate
7	Adenosine monophosphate
8	Adenosine diphosphate
9	Adenosine triphosphate
10	Flavin adenine dinucleotide
11	Nicotinamide adenine dinucleotide
12	Nicotinamide adenine dinucleotide, reduced
13	Nicotinamide adenine dinucleotide phosphate
14	Nicotinamide adenine dinucleotide phosphate, reduced

15	Erythrose 4-phosphate
16	Ribulose 5-phosphate / Xylulose 5-phosphate / ribose-5-phosphate combined
17	6-phosphogluconate
18	Sedoheptulose 7-phosphate
19	Fructose-6-phosphate + glucose-6-phosphate
20	Fructose-bisphosphate
21	Alanine
22	Asparagine
23	Aspartate
24	N-Acetyl-glucosamine-1-phosphate
25	Creatinine*
26	Gluconate
27	Glutamate
28	Glutamine
29	Glutathione, oxidized (GSSG)
30	Glutathione, reduced (GSH)

31	3-Phospho-serine
32	Serine
33	Threonine
34	Hexose (glucose etc.)
35	Histidine
36	Valine
37	Oleic acid
38	Palmitic acid
39	Stearic acid
40	Lysine
41	Methionine
42	Taurine
43	UDP-D-glucose
44	UDP-D-glucuronate
45	UDP-N-acetyl-D-glucosamine

46	Acetylphosphate*
47	Pantothenate*
48	Phenylalanine
49	Tyrosine
50	Inosine 5′-monophosphate (IMP)
51	Inosine
52	Xanthine
53	Guanosine diphosphate
54	Cytidine monophosphate
55	Uridine 5′-diphosphate (UDP)
56	Uridine 5′-monophosphate (UMP)
57	Uridine 5′-triphosphate (UTP)
58	Tryptophan

糖酵解 / 三羧酸等相关物质分析服务列表（4 种物质，Gly/TCA (Supplement to Central Metabolism Profile)) All analytes and Internal Standards are measured by EI on a GC-MS
1	Alpha Ketoglutarate
2	Fumurarte
3	Lactate
4	Pyruvate

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在技术报告中，百泰派克会为您提供详细的中英文双语版技术报告，报告包括：

1.实验步骤（中英文）
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3.鉴定物质的定量或定量信息
4.质谱图片
5.原始数据

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